gt1 infection Search Results


t gondii gt1  (ATCC)
93
ATCC t gondii gt1
<t>Toxoplasma</t> <t>gondii</t> strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for <t>GT1</t> and each of the other T. gondii strains.
T Gondii Gt1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gt1+infection/Toxoplasma+gondii+(Nicolle+and+Manceaux)+Nicolle+and+Manceaux/pmc04936363-48-0-3
Average 93 stars, based on 1 article reviews
t gondii gt1 - by Bioz Stars, 2026-10
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hcv gt1 infection  (Gilead Sciences)
99
Gilead Sciences hcv gt1 infection
<t>Toxoplasma</t> <t>gondii</t> strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for <t>GT1</t> and each of the other T. gondii strains.
Hcv Gt1 Infection, supplied by Gilead Sciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gt1+infection/Epclusa/pm27654843-47-24-32
Average 99 stars, based on 1 article reviews
hcv gt1 infection - by Bioz Stars, 2026-10
99/100 stars
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hepatitis c virus hcv genotype gt 1 infection  (Gilead Sciences)
97
Gilead Sciences hepatitis c virus hcv genotype gt 1 infection
<t>Toxoplasma</t> <t>gondii</t> strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for <t>GT1</t> and each of the other T. gondii strains.
Hepatitis C Virus Hcv Genotype Gt 1 Infection, supplied by Gilead Sciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gt1+infection/Harvoni/10__1002_slash_hep__28800-5748-57-69
Average 97 stars, based on 1 article reviews
hepatitis c virus hcv genotype gt 1 infection - by Bioz Stars, 2026-10
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dasabuvir exviera  (AbbVie Inc)
90
AbbVie Inc dasabuvir exviera
Patient characteristics
Dasabuvir Exviera, supplied by AbbVie Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gt1+infection/dasabuvir+exviera/pmc05552846-103-4-10
Average 90 stars, based on 1 article reviews
dasabuvir exviera - by Bioz Stars, 2026-10
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Image Search Results


Toxoplasma gondii strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for GT1 and each of the other T. gondii strains.

Journal: Infection and Immunity

Article Title: Functional Analysis of the Role of Toxoplasma gondii Nucleoside Triphosphate Hydrolases I and II in Acute Mouse Virulence and Immune Suppression

doi: 10.1128/IAI.00077-16

Figure Lengend Snippet: Toxoplasma gondii strain-dependent inhibition of luminescence in IFN-γ-inducible FLUC reporter lines. A tandem GAS element-driven FLUC reporter was stably expressed in HeLa cells (A) or RAW264.7 macrophages (B). Reporter cell lines were plated in 96-well plates and infected with different MOIs, as indicated. At 2 h after infection, the cells were stimulated with 100 U of IFN-γ/ml for 18 h. Subsequently, the cells were lysed, and the luciferase activity was measured. The data shown are means ± standard errors of the mean (SEM) of at least three independent experiments, each with three technical replicates. Kruskal-Wallis test with Dunn's correction (***, P < 0.0001) was performed for GT1 and each of the other T. gondii strains.

Article Snippet: T. gondii GT1 (ATCC 50853; type I), ME49 (ATCC 50611; type II), and CTG (ATCC 50842; type III) were used as representative type strains.

Techniques: Inhibition, Stable Transfection, Infection, Luciferase, Activity Assay

Mapping of a single target locus on chromosome XII. (A) GT1 (type I) and ME49 (type II) parental strains and 36 progeny were tested for their ability to interfere with the GAS-FLUC in RAW264.7 macrophages. The data shown are normalized averages of three technical replicates ± standard deviations (SD). (B and C) QTL scan of normalized percent inhibition identified a single locus of ∼688 kb (TGME49_chrXII: 6134501.0.6822749) on chromosome XII defined by the two markers 181 and 193 and the progeny SF50 and SF34. Fine mapping with additional PCR markers (see Table S2 in the supplemental material) within the QTL identified two additional informative progeny (2C7C3 and SF59) and narrowed the region responsible for the observed strain difference to ∼263 kb (TGME49_chrXII: 6559896.0.6822749) comprising 36 annotated genes.

Journal: Infection and Immunity

Article Title: Functional Analysis of the Role of Toxoplasma gondii Nucleoside Triphosphate Hydrolases I and II in Acute Mouse Virulence and Immune Suppression

doi: 10.1128/IAI.00077-16

Figure Lengend Snippet: Mapping of a single target locus on chromosome XII. (A) GT1 (type I) and ME49 (type II) parental strains and 36 progeny were tested for their ability to interfere with the GAS-FLUC in RAW264.7 macrophages. The data shown are normalized averages of three technical replicates ± standard deviations (SD). (B and C) QTL scan of normalized percent inhibition identified a single locus of ∼688 kb (TGME49_chrXII: 6134501.0.6822749) on chromosome XII defined by the two markers 181 and 193 and the progeny SF50 and SF34. Fine mapping with additional PCR markers (see Table S2 in the supplemental material) within the QTL identified two additional informative progeny (2C7C3 and SF59) and narrowed the region responsible for the observed strain difference to ∼263 kb (TGME49_chrXII: 6559896.0.6822749) comprising 36 annotated genes.

Article Snippet: T. gondii GT1 (ATCC 50853; type I), ME49 (ATCC 50611; type II), and CTG (ATCC 50842; type III) were used as representative type strains.

Techniques: Inhibition

Growth, virulence, and interference with STAT1 pathway. (A) Plaque assay on HFF monolayers examining growth of wild-type and NTPase knockout parasites. (B) Virulence of NTPase knockout strains in CD-1 mice infected intraperitoneally with 200 tachyzoites per mouse. Groups of five mice per strain were infected, and the survival was monitored. The experiment was repeated once, and the results of both experiments were plotted together. (C) A fluorescence assay performed to examine the interference of wild-type T. gondii with IRF1 protein expression as a readout for STAT1-activated transcription compared to NTPase knockout strains showed no NTPase-dependent effect. HFF monolayers were infected for 2 h and subsequently activated with 100 U of IFN-γ/ml for 5 h. Quantification of the IRF1 fluorescence of infected cells versus controls. Data points represent the mean red fluorescence in a ROI overlaying the host cell nucleus. IRF1, red; DAPI, blue; T. gondii-GFP, green. Scale bar, 10 μm. ***, P < 0.0001 (Kruskal-Wallis with Dunn's correction).

Journal: Infection and Immunity

Article Title: Functional Analysis of the Role of Toxoplasma gondii Nucleoside Triphosphate Hydrolases I and II in Acute Mouse Virulence and Immune Suppression

doi: 10.1128/IAI.00077-16

Figure Lengend Snippet: Growth, virulence, and interference with STAT1 pathway. (A) Plaque assay on HFF monolayers examining growth of wild-type and NTPase knockout parasites. (B) Virulence of NTPase knockout strains in CD-1 mice infected intraperitoneally with 200 tachyzoites per mouse. Groups of five mice per strain were infected, and the survival was monitored. The experiment was repeated once, and the results of both experiments were plotted together. (C) A fluorescence assay performed to examine the interference of wild-type T. gondii with IRF1 protein expression as a readout for STAT1-activated transcription compared to NTPase knockout strains showed no NTPase-dependent effect. HFF monolayers were infected for 2 h and subsequently activated with 100 U of IFN-γ/ml for 5 h. Quantification of the IRF1 fluorescence of infected cells versus controls. Data points represent the mean red fluorescence in a ROI overlaying the host cell nucleus. IRF1, red; DAPI, blue; T. gondii-GFP, green. Scale bar, 10 μm. ***, P < 0.0001 (Kruskal-Wallis with Dunn's correction).

Article Snippet: T. gondii GT1 (ATCC 50853; type I), ME49 (ATCC 50611; type II), and CTG (ATCC 50842; type III) were used as representative type strains.

Techniques: Plaque Assay, Knock-Out, Infection, Fluorescence, Expressing

Patient characteristics

Journal: Wiener Klinische Wochenschrift

Article Title: Progress in eradication of HCV in HIV positive patients with significant liver fibrosis in Vienna

doi: 10.1007/s00508-016-1162-y

Figure Lengend Snippet: Patient characteristics

Article Snippet: In the case of HCV-GT1 infection, dasabuvir (Exviera® 250 mg, Abbvie, Vienna, Austria) twice daily was added.

Techniques: Infection, Transmission Assay